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The purpose of this research will be to characterize the metabolites of EAM-2201 in human hepatocytes obtained in vitro and those in liver and urine specimens obtained click here in vivo through the autopsy of the EAM-2201 abuser.
Twelve metabolites of a artificial cannabinoid, EAM-2201, have been tentatively recognized in human hepatocytes in vitro, and in human liver and urine specimens in vivo for The 1st time. There was a distinct big difference in metabolism profile in between the in vitro As well as in vivo effects.
EAM-2201 was incubated with human hepatocytes for three h in the CO2 incubator as well as the metabolites of EAM-2201 were manufactured. The human liver specimen was homogenized along with the metabolites were extracted. The urine specimen was hydrolyzed initially with β-glucuronidase along with the metabolites were extracted.
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